Detection and clinical characteristics analysis of human Bocavirus 1-3 in children for acute respiratory infection in Lanzhou area
10.3760/cma.j.issn.1003-9279.2011.01.003
- VernacularTitle:兰州地区呼吸道感染患儿中人类博卡病毒1～3型检测与临床研究
- Author:
Chang-Qing CAO
1
;
Yu-Ning LI
;
Yu JIN
;
Zhi-Ping XIE
;
Han-Chun GAO
;
Qiong-Hua ZHOU
;
Xiao-Qian GAO
;
Ya-Ting ZHANG
;
Jian ZHANG
;
Zhao-Jun DUAN
Author Information
1. 兰州大学第一医院儿科
- Keywords:
Respiratory infections;
Bocavirus 1-3,human;
Polymerase chain reaction
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(1):5-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the clinical and molecular epidemiology characteristics of human Bocavirus 1-3 (HBoV1-3) in children for acute respiratory infection in Lanzhou area. Methods Nasopharyngeal aspiration samples and throat swabs were collected from 524 children with ARTI at the First Hospital of Lanzhou University,Gansu Province,China,between December 2009 and November 2010. Nested PCR was employed to screening HBoV1-3, which amplified a 518-bp fragment of the partial NS1 gene.Furthermore, a standard reverse transcription-PCR was used to screen for other common respiratory viruses.Results The overall frequency of H BoV was 8.2% (43/524), lining up behind human rhinovirus, RSV,parainfluenza virus 3. Thirty of the HBoV-postive children (69. 8% ) were co-infected with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously higher than that in AURI. The 2 HBoV2 NS1 sequences shared 99% and 100% nucleotide sequence identity with HBoV2 strain CU47TH respectively.Two cases of HBoV2 postive children appears gastrointestinal symptoms. The one HBoV3 NS1 sequences shared 99% nucleotide sequence identity with HBoV3 isolate 46-BJ07. Conclusion The HBoV3 was detected at the first time in lanzhou area. HBoV1-3 infection exists in children with acute respiratory tract infections in Lanzhou region, HBoV1 were dominant. The mixed infection rate was higher.
