The study on frequency distribution of regulatory T cells and its functional markers in peripheral blood of chronic hepatitis B
10.3760/cma.j.issn.1003-9279.2011.01.012
- VernacularTitle:慢性乙型肝炎患者外周血调节性T细胞频率分布及功能标记的研究
- Author:
Chen LI
1
;
Shao-Jun XING
;
Xue-Zhang DUAN
;
Mo-Bin WAN
;
Hui-Fen WANG
Author Information
1. 第二军医大学长海医院感染科
- Keywords:
Hepatitis B chronic;
T-Lymphocytes;
Functional marker
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(1):33-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of treg cells and its functional markers in pathogenesis of chronic hepatitis B, and the correlation with disease progression. Methods 20 cases of healthy control people,53 cases of chronic hepatitis B patients and 24 cases of liver cirrhosis patients were enrolled into the groups. Detecting the frequencies of CD4 + CD25 + Fox3 + cells, CD4 + CD25 + CD127low cells, CD39 + treg cells and CTLA-4+ treg cells in treg cells by flow cytometry. Clinical parameters were investigated in the same time. Results The frequencies of treg cells, CD4 + CD25 + CD127low cells and CD39 + treg cells were significant different among healthy control group,CHB group and LC group( P ＜ 0.01 ). The frequencies of treg cells,CD4 + CD25 + CD127low cells and CD39 + treg cells were significantly different in moderate-severe CHB group compared with mild CHB group(P ＜0.05 ,P ＜0.05 ,P ＜ 0. 01 ). In CHB group the frequencies of CD4 + CD25 + Foxp3 + cells were positively correlated with ALT ( r = 0. 289, P ＜ 0.05 ) and AST ( r =0. 302,P ＜0.05), the frequencies of CD4+ CD25 + Foxp3 + cells had a significant positive correlation with the frequencies of CD4 + CD25 + CD127low cells(r = 0.478,P ＜ 0. 01 ). Conclusion The frequencies of treg cells and its functional markers probably had a dynamic tendency in the process of chronic hepatitis B and were closely related with the change of liver function parameters. CD39 + treg cells may be a group of functional treg cells,which indicated that CD39 be a sensitive marker to react treg cells function. In some sense, CD4 + C D25 + CD 127 low cells frequency could represent treg cell frequency.
