Molecular and epidemiological study on viral diarrhea among infants in Lanzhou
10.3760/cma.j.issn.1003-9279.2011.01.013
- VernacularTitle:兰州地区5岁以下婴幼儿病毒性腹泻的分子流行病学研究
- Author:
Xuan LUO
1
;
Yu JIN
;
Yu-Ning LI
;
Jin-Song LI
;
Wei-Xia CHENG
;
Ye-Xia HAO
;
Yong-Xia WANG
;
Shu-Xian CUI
;
Zhao-Jun DUAN
Author Information
1. 兰州大学第一医院
- Keywords:
Diarrhea,infection;
Child,preschool;
Epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(1):36-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the epidemiologic characteristics of viral diarrhea in children under 5 years old in Lanzhou, understand the four major virus in children of distribution. Methods In the first hospital of Lanzhou university from Jul 2009 to Jun 2010 ,we collected 290 stool specimens from children with diarrhea and 114 asymptomatic controls. Rotavirus was detected by ELISA, further strain characterization was carried out by nested PCR. The human calicivirus, astrovirus, adenovirus were detected by RT- multiplex PCR and PCR. Results At least one of the four viral agents was found in 60% of the specimens.Rotavirus, human calicivirus, adenovirus, and astrovirus were identified in 39. 31% , 11.38 % , 10.69% , and 4.83% in 290 specimens respectively. Rotavirus G3 was the most prevailing serotype, P [8] was the most common genotype. In the 114 control samples, 7 sample was positived for calicivirus, 5 samples were positived for human adenovirus and 1 sample was positived for astrovirus. Conclusion The results indicated clearly the impact of viral agents causing diarrhea and the importance of long-term systematic surveillance.
