Ananlysis on the molecular characteristics of CVA9 virus in Tibet

VernacularTitle:西藏自治区1999-2000年儿童柯萨奇病毒A组9型分子流行病学分析
Author: Xin-Xin ZHANG ¹ ; Li CHEN ; Hui-Ling WANG ; Meng-Nan JIANG ; Hui ZHU ; Wen-Bo XU ; Xiao-Hui KONG
Author Information

1. 首都医科大学附属北京儿童医院病毒室
Keywords: Coxsackievirus infeclions; Genes
From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):42-45
CountryChina
Language:Chinese
Abstract: Objective To study the molecular characterization of CVA9 virus in Tibet. Methods To isolate the enteroviruses from stool specimens of AFP cases and other children in Tibet in 1999-2002, and identify them by neutralization test using the RIVM antiserum; then determine the complete nucleotide sequence of VP1 region of CVA9 viruses, and analyze the results. Results A total of 10 strains of CVA9virus were isolated from the stool specimens and identified. The complete nucleotide sequence of VP1 region of these CVA9 viruses were 906nt coding 302 amino acids. To compare with the sequences of the 10 strains of Tibet, the homogeneity of nucleotide sequence were 79.0% -99.9%; while they were 75.7% -78.7%compared with Griggs. The phylogenetic tree of CVA9 viruses showed 2 groups, and the isolates from Tibet belong to 1, 2 groups. Conclusion The deduction is that the 10 strains are proposed 2 different groups, the strains epidemic in 1999 belong to group 2,while strains in 2000 belong to group 1.