Detection and molecular characterization of human parechovirus (HPeV) in children with acute gastroenteritis.
- Author:
Jie LI
1
;
Qing ZHANG
;
Zi-qian XU
;
Wei-xia CHENG
;
Dan-di LI
;
Hui-ying LI
;
Jin-xin XIE
;
Shu-xian CUI
;
Na LIU
;
Jing-bo ZHAO
;
Zhao-jun DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Child, Preschool; Female; Gastroenteritis; epidemiology; virology; Humans; Infant; Male; Parechovirus; classification; genetics; isolation & purification; Phylogeny
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):46-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study HPeV from stool samples of children with acute gastroenteritis under 5 years old.
METHODSWe conducted a real-time PCR to detect HPeV from stool samples and to amply VP1 sequence by nested RT-PCR to identify HPeV type.
RESULTSThe results showed that 27 of 306 (8.82%) children with acute gastroenteritis were infected HPeV. 11 strains were typed. 9 strains HPeV1, both HPeV2 and HPeV4 was 1 strain. HPeV was mostly identified in autumn season with a peak in July. HPeV seemed relevant in children >2 years old. The range of nucleotide identity between all isolated strains with reference strains was 79%-92%.
CONCLUSIONEpidemiology characteristic of HPeV in Jilin was concordance with that of reports. HPeV3 wasnt detected. It's significant to conduct the large scale and long-term surveillance of HPeV.
