Detection and molecular characterization of human parechovirus (HPeV) in children with acute gastroenteritis

VernacularTitle:人双埃柯病毒在腹泻患儿中的流行及其分子特征研究
Author: Jie LI ¹ ; Qing ZHANG ; Zi-Qian XU ; Wei-Xia CHENG ; Dan-Di LI ; Hui-Ying LI ; Jin-Xin XIE ; Shu-Xian CUI ; Na LIU ; Jing-Bo ZHAO ; Zhao-Jun DUAN
Author Information

1. 哈尔滨医科大学公共卫生学院
Keywords: Parechovirus; Diarrhea
From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):46-48
CountryChina
Language:Chinese
Abstract: Objective To study HPeV from stool samples of children with acute gastroenteritis under 5 years old. Methods We conducted a real-time PCR to detect HPeV from stool samples and to amply VP1 sequence by nested RT-PCR to identify HPeV type. Results The results showed that 27 of 306 (8.82%) children with acute gastroenteritis were infected HPeV. 11 strains were typed. 9 strains HPeV1,both HPeV2 and HPeV4 was 1 strain. HPeV was mostly identified in autumn season with a peak in July.HPeV seemed relevant in children ＞ 2 years old. The range of nucleotide identity between all isolated strains with reference strains was 79%-92%. Conclusion Epidemiology characteristic of HPeV in Jilin was concordance with that of reports. HPeV3 wasnt detected. It's significant to conduct the large scale and longterm surveillance of HPeV.