Enterovirus 71 induces apoptosis in a Bax dependent manner.
- Author:
Zhen-min SUN
1
;
Yan XIAO
;
Li-li REN
;
Xiao-bo LEI
;
Jian-wei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspases; physiology; Cell Line, Tumor; Enterovirus A, Human; pathogenicity; Humans; Rhabdomyosarcoma; pathology; virology; bcl-2-Associated X Protein; physiology
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular mechanism of apoptosis induced by enterovirus 71 (EV71) infection.
METHODSThe effects of EV71 on Rhabdomyosarcoma (RD) cell viability were detected by CCK8 assay. EV71-induced apoptosis on RD cells were detected by Hoechst 33342 staining and Western blot targeting Caspase 3, 8 and PARP. Bax conformational change was detected by immunoprecipitation with Bax 6A7 antibody.
RESULTSEV71 decreased the viability of RD cells and induces the activation of Caspase 3, 8 and PARP. Bax expression increases in RD cells after EV71 infection, and Bax conformational change also can be detected after EV71 infection.
CONCLUSIONOur study reveals that EV71 induces Caspase-dependent apoptosis by Bax conformational change.
