Enterovirus 71 induces apoptosis in a Bax dependent manner

VernacularTitle:肠道病毒71型诱导Bax依赖的细胞凋亡
Author: Zhen-Min SUN ¹ ; Yan XIAO ; Li-Li REN ; Xiao-Bo LEI ; Jian-Wei WANG
Author Information

1. 北京协和医学院/中国医学科学院病原生物学研究所病毒基因工程国家重点实验室
Keywords: Enterovirus; Apoptosis
From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):49-52
CountryChina
Language:Chinese
Abstract: Objective To explore the molecular mechanism of apoptosis induced by enterovirus 71 ( EV71 ) infection. Methods The effects of EV71 on Rhabdomyosarcoma (RD) cell viability were detected by CCK8 assay. EV71-induced apoptosis on RD cells were detected by Hoechst 33342 staining and Western blot targeting Caspase 3, 8 and PARP. Bax conformational change was detected by immunoprecipitation with Bax 6A7 antibody. Results EV71 decreased the viability of RD cells and induces the activation of Caspase 3, 8 and PARP. Bax expression increases in RD cells after EV71 infection, and Bax conformational change also can be detected after EV71 infection. Conclusion Our study reveals that EV71 induces Caspasedependent apoptosis by Bax conformational change.