Identification A Novel protein TRIM38 that activate NF-κB Signaling Pathways

VernacularTitle:TRIM38蛋白对核转录因子NF-κB的影响
Author: Xin-Lei LIU ¹ ; Xiao-Bo LEI ; Jian-Wei WANG ; Tao HONG
Author Information

1. 北京协和医学院/中国医学科学院病原生物学研究所病毒基因工程国家重点实验室
Keywords: TRIM38; Transcription factor; Transcription,genetic; Grenes
From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):60-62
CountryChina
Language:Chinese
Abstract: Objective The tripartite motif (TRIM) proteins are a family of more than 70 human members, however only a few of them have been well studied. It has been shown that TRIM proteins are involved in various cellular processes such as cell proliferation, differentiation, apoptosis and antiviral defense. The functions of TRIM38 are largely unknown. In this study we explore the effect of TRIM38 on NF-κB signaling pathway. Methods 293T cells were transfected with NF-κB-Luc and plasmids expressing TRIM38 and its mutants fused to Flag. 24 h after transfection, cells were harvested and luciferase activities were measured. Data are representative of three independent experiments with triplicate samples. The expression of proteins was analyzed by Western Blot. Results TRIM38 could activate NF-κB signaling pathway. The mutants of TRIM38 affected the function of TRIM38. Only the mutant of SPRY domain deletion had no obviously influence of the function of TRIM38. Conclusion Our study reveals that NF-κB is activated in response to TRIM38.