Compare real-time RT-PCR with two culture methods for influenza virus detection

VernacularTitle:荧光RT-PCR法及两种培养法检测流感病毒的比较分析
Author: Jian-Xiong LI ¹ ; Shi-Song FANG ; Xiao-Wen CHENG ; Ting WANG ; Xin WANG ; Xing LV ; Chun-Li WU ; Ren-Li ZHANG ; Jin-Quan CHENG ; Mu-Hua YU
Author Information

1. 中山大学公共卫生学院
Keywords: Influenza virus; Polymerase chain reaction; Cell culture; Chink embryo
From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):66-68
CountryChina
Language:Chinese
Abstract: Objective Real-time RT-PCR, cell culture and embryonated eggs culture for influenza detection were compared by analyzing the data of influenza surveillance in Shenzhen in second half of 2009.Methods 1092 clinical samples ( throat swabs ) collected during second half of 2009 were tested by realtime RT-PCR, cell culture and embryonated eggs culture, and the results were analyzed by statistical methods. Results The positive rate were 54. 21% ,27.11% and 16. 21% using real-time RT-PCR, cell culture and embryonated eggs culture, and the sensitive were 100% ,50% and 29. 9%. The lowest dilutions of virus detected by real-time RT-PCR were 10-2TCID50/ml. Conclusion The sensitive of real-time RTPCR was higher than culture and the specificity was also very high. It was more suitable for emergency detect. The sensitive of cell culture for H3N2 subtype was higher, and sensitive of embryonated eggs culture for type B was higher.