Detection and typing of human papillomavirus by a GeXP based multiplex PCR assay.
- Author:
Chun-bin LU
1
;
Meng-jie YANG
;
Le LUO
;
Miao WANG
;
Xue-jun MA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Female; Humans; Middle Aged; Papillomaviridae; classification; genetics; isolation & purification; Polymerase Chain Reaction; methods
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(1):69-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new and rapid GeXP based multiplex PCR assay for the detection and typing of human papillomavirus 6, 11, 31, 33 and 52.
METHODSNucleotide sequences of HPV6, HPV11, HPV31, HPV33 and HPV52 from NCBI were obtained and compared. Genotype-specific primers were then designed and the sensitivity and specificity of multiple PCR assay was evaluated. Optimized assay was further validated with 30 clinical specimens collected from the cervical secretions of patients.
RESULTSA GeXP based multiplex PCR was developed for sensitive detection and reliable differentiation of five HPV genotypes (HPV6, 11, 31, 33 and 52),
CONCLUSIONA GeXP based multiplex PCR assay is demonstrated to be a new and rapid technique for simultaneous detection and typing of 5 different human papillomaviruses.
