Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening

VernacularTitle:机会性筛查人群中高危型人乳头瘤病毒与宫颈病变相关性的分析
Author: Li-Hua BIAN ¹ ; Ying LI ; Xiao-Li WANG ; Xiao-Zhou WU ; Lei SONG ; Hong-Tu LIU
Author Information

1. 解放军总医院
Keywords: Hybridization; Papillomavirus,human; Genotyping; Cytological techniques
From: Chinese Journal of Experimental and Clinical Virology 2011;25(2):89-91
CountryChina
Language:Chinese
Abstract: Objective To investigate the association between HPV genotypes and cervical lesion in Hybrid Capture 2 ( HC2 ) HPV test positive samples. Methods 602 cervical samples randomly obtained detected as HPV positive by the HC2 high-risk probe cocktail were determined by polymerase chain reaction (PCR)-reverse dot blot (RDB), among them 344 participated Thinprep Cytology test. Results 569 (94. 5% ) samples were successfully amplified. The most common HPV genotypes were HPV16(31.6% ),52 ( 16. 7 % ), 58 ( 15.1% ), 56 ( 8. 1% ), 39 ( 7. 9% ) ; HPV 16 or HPV 16/18 infection was significantly related to ASCUS, LSIL and HSIL; 24 samples suggested single HPV infection with the genotypes not available for HC2. Conclusion The most common HPV genotypes in random screening were HPVI6, 52 and HPV58; Our data demonstrated the pseudo-positivity of HC2 test was 4.0% ; HPV16 was a index for progression of HSIL.