The analysis of IL-10 and its methylation in the patients with acute on chronic liver failure.
- Author:
Zhao-xia QI
1
;
Shu-xia YU
;
Hong-sheng HAO
;
Feng-cai LI
;
Zhao-yang GUO
;
Yu-chen FAN
;
Kai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Chronic Disease; DNA Methylation; Female; Humans; Interleukin-10; blood; genetics; metabolism; Liver Failure, Acute; blood; genetics; metabolism; Male; Methylation; Middle Aged; Promoter Regions, Genetic; Young Adult
- From:Chinese Journal of Experimental and Clinical Virology 2011;25(2):99-101
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of IL-10 and the methylation of its promoter in acute on chronic liver failure (ACLF).
METHODSPatients were divided into three groups: 25 with ACLF, 25 with CHB, 10 healthy controls. Respectively detect the serum level of IL-10 via ELISA, and the methylation of IL-10 promoter via MSP, to analyze the difference among the three groups.
RESULTSBoth the ACLF group and the CHB group have significant increase in serum level of IL-10 compared with the control group (P < 0.05); the ACLF group's level is higher than the CHB group, however without statistical significance (P > 0.05). The serum level of IL-10 in ACLF group has no significant relativity with ALT and HBV-DNA( r = -0.022, r = 0.033, respectively; P > 0.05); has positive relativity with TBIL and MELD ( r = 0.566, r = 0.443, respectively; P < 0.05); and negative relativity with PTA (r = -0.581, P < 0.05). The distribution of the methylation of IL-10 promoter in ACLF group is significantly different from the other two.
CONCLUSIONThe serum level of IL-10 in hepatitis patients is significantly higher and increases with the degree of liver failure. The promoter methylation may be important in the gene inactivation.