The analysis of IL-10 and its methylation in the patients with acute on chronic liver failure
10.3760/cma.j.issn.1003-9279.2011.02.006
- VernacularTitle:慢加急性肝衰竭中IL-10的表达及其启动子甲基化状态的研究
- Author:
Zhao-Xia QI
1
;
Shu-Xia YU
;
Hong-Sheng HAO
;
Feng-Cai LI
;
Zhao-Yang GUO
;
Yu-Chen FAN
;
Kai WANG
Author Information
1. 山东大学齐鲁医院
- Keywords:
Interleukin-10;
Hepatitis B;
Methylation;
Liver failure
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(2):99-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of IL-10 and the methylation of its promoter in acute on chronic liver failure (ACLF). Methods Patients were divided into three groups: 25 with ACLF, 25 with CHB, 10 healthy controls. Respectively detect the serum level of IL-10 via ELISA, and the methylation of 1L-10 promoter via MSP,to analyze the difference among the three groups. Results Both the ACLF group and the CHB group have significant increase in serum level of IL-10 compared with the control group ( P <0.05 ) ; the ACLF group's level is higher than the CHB group, however without statistical significance (P >0.05 ). The serum level of IL-10 in ACLF group has no significant relativity with ALT and HBV-DNA( r = -0. 022, r = 0. 033, respectively; P > 0. 05 ) ; has positive relativity with TBIL and MELD ( r = 0. 566, r =0.443, respectively; P < 0.05); and negative relativity with PTA (r = - 0.581, P < 0.05). The distribution of the methylation of IL-10 promoter in ACLF group is significantly different from the other two.Conclusion The serum level of IL-10 in hepatitis patients is significantly higher and increases with the degree of liver failure. The promoter methylation may be important in the gene inactivation.
