Adenoviruses mediated BCL-Xl overexpression protects mice from fulminant hepatic failure
10.3760/cma.j.issn.1003-9279.2011.02.009
- VernacularTitle:重组腺病毒介导的BCL-Xl基因过表达治疗急性肝衰竭大鼠的研究
- Author:
Xiao-An YANG
1
;
Ka ZHANG
;
Xin SHU
;
Hong CAO
;
Qi-Huan XU
Author Information
1. 中山大学附属第三医院
- Keywords:
Liver failure;
Gene therapy;
Genes,BCL-Xl;
Apoptosis;
Adenoviridae
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(2):109-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To indentify the relation between hepatic ceils apoptosis and the lesion of liver tissue in acute toxic lethal hepatitis. Methods 60 Wistar mice were randomly divided into normal control, model group and treatment group. Normal control and model group were pretreated by portal vein injection of normal saline, the treatment group was pretreated by portal vein injection of BCL-Xl adenoviruses. The mice of model group and treatment group were received an injection of D-gain and LPS to establish fulminant hepatic failure models 7 days after pretrement. To observe BCL-Xl expression, serum ALT,AST, hepatocyte apoptosis rate, and mortality rate of the three groups. Results The BCL-Xl expression was higher in treatment group than in model group ;6 hours after fulminant hepatic failure models were established, the serum ALT, AST level of treatment group was lower than model group; The hepatoeyte apoptosis rate of treatment group was lower than model group. The death rate of treatment group was lower than model group. Conclusion In fulminant mice hepatic failure models,the hepatocyte apoptosis rate has a positive correlation with death rate, the overexpression of BCL-Xl can decrease the hepatocyte apoptosis rate and the death rate.
