Distribution of hepatitis B virus genotypes and serotypes in people who had a physical examination in Yunnan Province
10.3760/cma.j.issn.1003-9279.2011.02.011
- VernacularTitle:云南省乙型肝炎病毒基因型和血清型的流行状况分析
- Author:
Wen-Yu KANG
1
;
Zheng-Rong DING
;
Li-Ping SHEN
;
Zhi-Xian ZHAO
;
Bing-Jun TIAN
;
Hui LI
;
Yong LI
;
Shuang ZHANG
;
Sheng-Li BI
Author Information
1. 云南省疾病预防控制中心
- Keywords:
Hepatitis B virus;
Sequencing analysis;
Genotype;
Serotyping
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(2):114-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To know genotypes and serotypes of hepatitis B virus ( HBV ) detected from hepatitis B infected people in Yunnan Province. Methods Serum samples were collected from HBsAg carriers detected from people who had a physical examination at Yunnan Provincial Center for Disease Control and Prevention. The S genes of HBV were amplified by nested PCR and the PCR products were sequenced.The viral genotype was identified by phylogenetic analysis. 27 reference sequences corresponding to HBV genotype A to I were obtained from GenBank. According to the amino acid sequences deduced from the nucleotide sequences of S gene, the dominant serotype of HBV detected from these people were confirmed.Results 39 HBsAg positive serum samples were detected from 2216 people who had a physical examination. The results shows that 76.9% were C genotype; 15.4% were B genotype;5.1% were D genotype;2. 5% were I genotype. Three serotypes were found. The rates of adw2, adrq+ and ayr serotypes are 71.8% ,17.9% and 10. 3% respectively. All of adw2 subtype specimens are C genotype. Among the serum specimens in which both HBsAg and HBeAg are positive, 75% were C genotype and adw2 subtype.Conclusion It is determined that the main genotype and subtype of HBV prevailed in Yunnan province is C genotype and adw2 subtype.
