Construction and identification of attenuated Salmonella which harboring enterovirus 71 VP1 gene.
- Author:
Ze LIU
1
;
Yan HU
;
Hong-hui SHEN
;
Shao-ping CAI
;
Bing-ke BAI
;
Rong GAO
;
Sheng-dong LUO
;
Yan-tao CHAI
;
Pan-yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Capsid Proteins; genetics; metabolism; Enterovirus A, Human; genetics; Gene Expression; Genetic Engineering; Genetic Vectors; genetics; metabolism; Salmonella; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(2):117-119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop attenuated Salmonella which harboring enterovirus 71 (EV71) VP1 gene.
METHODSThe plasmid which expressed VP1 protein of EV71 was constructed by gene recombination. Cellular expression was assessed by Western Blot analysis. The recombinant plasmid was then transformed into attenuated Salmonella SL7207.
RESULTSEV71 VP1 gene sequence was inserted into a eukaryotic expression plasmid VR1012. VP1 protein was detected by Western Blot analysis in the culture supernatant. And the attenuated Salmonella harbored the plasmid stable.
CONCLUSIONThe plasmid was constructed successfully and it can express effectively in vitro. The bacteria which harboring the plasmid were constructed successfully. This has provided a basis for further research of an oral EV71 vaccine.