Construction and identification of attenuated Salmonella which harboring enterovirus 71 VP1 gene

VernacularTitle:携带肠道病毒71型VP1基因的重组减毒沙门菌的构建和鉴定
Author: Ze LIU ¹ ; Yan HU ; Hong-Hui SHEN ; Shao-Ping CAI ; Bing-Ke BAI ; Rong GAO ; Sheng-Dong LUO ; Yan-Tao CHAI ; Pan-Yong MAO
Author Information

1. 军医进修学院
Keywords: Enterovirus; Vaccine DNA; Salmonella typhi
From: Chinese Journal of Experimental and Clinical Virology 2011;25(2):117-119
CountryChina
Language:Chinese
Abstract: Objective To develop attenuated Salmonella which harboring enterovirus 71 (EV71) VPI gene. Methods The plasmid which expressed VP1 protein of EV71 was constructed by gene recombination. Cellular expression was assessed by Western Blot analysis. The recombinant plasmid was then transformed into attenuated Salmonella SL7207. Results EV71 VP1 gene sequence was inserted into a eukaryotic expression plasmid VR1012. VP1 protein was detected by Western Blot analysis in the culture supernatant. And the attenuated Salmonella harbored the plasmid stable. Conclusion The plasmid was constructed successfully and it can express effectively in vitro. The bacteria which harboring the plasmid were constructed successfully. This has provided a basis for further research of an oral EV71 vaccine.