The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD.
- Author:
Yan TANG
1
;
Jun HOU
;
Jun XU
;
Hong-hui SHENG
;
Wei-wei CHEN
;
Bo-an LI
;
Jun ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Child; Child, Preschool; DNA Primers; chemistry; genetics; Enterovirus A, Human; genetics; isolation & purification; Female; Fluorescent Dyes; chemistry; Hand, Foot and Mouth Disease; diagnosis; pathology; virology; Humans; Infant; Male; Polymerase Chain Reaction; methods
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(2):152-154
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the real-time fluorescent PCR method for detecting enterovirus, enterovirus 71 and Coxsackievirus A 16 nucleic acid.
METHODSPrimers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.
RESULTSThe real-time fluorescent PCR positive rates of EV, EV71 and Cox A16 were 73.7%, 60.5%, 13.2%; the conventional RT-PCR were 71.1%, 55.3%, 13.2%. There were no significant differences between the two methods.
CONCLUSIONThe real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.
