The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD

VernacularTitle:实时荧光PCR检测手足口病病原体方法的建立及初步应用
Author: Yan TANG ¹ ; Jun HOU ; Jun XU ; Hong-Hui SHENG ; Wei-Wei CHEN ; Bo-An LI ; Jun ZHAO
Author Information

1. 解放军军医进修学院
Keywords: Hand,foot and mouth disease; Enterovirus; Coxsackievirus infections; Reverse transcriptase polymerase chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2011;25(2):152-154
CountryChina
Language:Chinese
Abstract: Objective To establish the real-time fluorescent PCR method for detecting enterovirus,enterovirus 71 and Coxsackievirus A 16 nucleic acid. Methods Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.Results The real-time fluorescent PCR positive rates of EV, EV71 and Cox AI6 were 73.7% , 60. 5%,13. 2% ;the conventional RT-PCR were 71. 1%, 55.3%, 13.2%. There were no significant differences between the two methods. Conclusion The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.