Construction of vectors expressing M2 and NA genes of H5N1 influenza virus.
- Author:
Jian-Qiang GUO
1
;
Li-Hong YAO
;
Ai-Jun CHEN
;
Xiao-Yu LIU
;
Jin-Qi FU
;
Peng-Wei XU
;
Zhi-Qing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Genetic Vectors; genetics; Humans; Influenza A Virus, H5N1 Subtype; genetics; metabolism; Neuraminidase; genetics; metabolism; Plasmids; genetics; Viral Matrix Proteins; genetics; metabolism; Viral Proteins; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(3):167-169
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct vectors expressing M2 and NA genes of H5N1 influenza virus.
METHODSBased on the human H5N1 avian influenza virus (A/Anhui/1/2005) isolated in china, M2 and NA genes were amplified by PCR. M2 or NA gene was subcloned into pStar vector to construct recombinant pStar-M2/, pStar-/M2, pStar-NA/and pStar-NA/. Furthermore, both of the M2 and NA genes were subcloned into pStar to construct two genes co-expressing recombinant pStar-M2/NA and pStar-NA/M2. Expression of the genes were detected by IFA after transfection of 293 cells with the recombinant plasmids.
RESULTSRecombinant plasmids were constructed and identified by restriction endonuclease digestion. Expression of the genes cloned into the recombinant plasmids was confirmed by IFA.
CONCLUSIONRecombinant plasmids expressing M2 and/or NA genes of H5N1 influenza virus were constructed, which provided basis for development of influenza DNA vaccine.
