Effects of human cytomegalovirus on the cell cycle of duct epithelial cell cultures of human salivary gland in vitro and relative mechanism.
- Author:
Guo-Rong YANG
1
;
Gao-Sheng HUANG
;
Xiao-Xia HE
;
Yong-Jie XUE
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Culture Techniques; Cell Cycle; physiology; Cyclin D1; genetics; metabolism; Cytomegalovirus; physiology; Epithelial Cells; cytology; virology; Flow Cytometry; Humans; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Salivary Glands; cytology; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(3):188-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of human cytomegalovirus (HCMV) on the cell cycle of duct epithelial cell cultures of human salivary gland (HSG) in vitro and relative mechanism.
METHODSHSG was cultured in vitro. Reverse transcriptase polymerase chain reaction (RT-PCR) and nest-RT-PCR were used respectively to investigate ie1/ie2 transcription in HSG infected by human cytomegalovirus(HCMV). The effects of HCMV on the cell cycle of HSG were studied by flow cytometry in vitro. The expression of cyclin D1 in HSG infected by HCMV was detected by Western blotting.
RESULTSHCMV iel/ie2 transcription could be detected in HSG infected by HCMV. HCMV arrested productively infected cells in G1 stage. And cyclin D1 was down-regulated in HCMV infected HSG.
CONCLUSIONHCMV inhibits proliferation of HSG by affecting G1/S check point and down-regulating cyclin D1 in vitro.
