Effects of human cytomegalovirus on the cell cycle of duct epithelial cell cultures of human salivary gland in vitro and relative mechanism

VernacularTitle:人巨细胞病毒对体外培养的人涎腺导管上皮细胞周期的影响及其相关机制
Author: Guo-Rong YANG ¹ ; Gao-Sheng HUANG ; Xiao-Xia HE ; Yongjie XUE
Author Information

1. 解放军第一医院
Keywords: Cytomegalovirus; Salivary glands; Epithelial cells; Cell cycle
From: Chinese Journal of Experimental and Clinical Virology 2011;25(3):188-190
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of human cytomegalovirus (HCMV) on the cell cycle of duct epithelial cell cultures of human salivary gland ( HSG) in vitro and relative mechanism. Methods HSG was cultured in vitro. Reverse transcriptase polymerase chain reaction (RT-PCR) and nestRT-PCR were used respectively to investigate ie1/ie2 transcription in HSG infected by human cytomegalovirus( HCMV). The effects of HCMV on the cell cycle of HSG were studied by flow cytometry in vitro. The expression of cyclin D1 in HSG infected by HCMV was detected by Western blotting. Results HCMV ie1/ie2 transcription could be detected in HSG infected by HCMV. HCMV arrested productively infected cells in G1 stage. And cyclin D1 was down-regulated in HCMV infected HSG. Conclusion HCMV inhibits proliferation of HSG by affecting G1/S check point and down-regulating cyclin D1 in vitro.