The structure and function analysis of duplicate genes in Merlin strains of human cytomegalovirus
10.3760/cma.j.issn.1003-9279.2011.03.012
- VernacularTitle:人巨细胞病毒Merlin株全基因组的重叠基因结构与功能分析
- Author:
Guang YANG
1
;
Yue-Qin LI
;
Yi ZOU
;
Xin ZHANG
;
Tian-Hong ZHOU
Author Information
1. 暨南大学
- Keywords:
Cytomegalo virus;
Genome;
Bioinformatics
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(3):194-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the genes in which exist overlapping ORF in Merlin strains of human cytomegalovirus, and to reveal their structure and functional characteristics. Methods We search for overlapping genes of ORF in HCMV Merlin strains' whole genome by Bioinformatics methods, analyzing coding sequence CDS and starting and ending sites of ORF, calculating the length of CDS and ORF, analyzing the molecular weight of encoding protein, overlapping length and coding direction of protein, identifying overlapping sequences and overlapping types, analyzing the expression phase of overlapping genes and the function of proteins. Results There were 39 overlapping ORF genes in HCMV Merlin strains, accounting for 23% of total genes. Among these 39 genes, there are 13 IE genes, 9 E genes and 17 L genes, which can be divided into 16 contigs. There are 11 contigs when two genes overlap, with 3 contigs in three genes overlapping, and 2 contigs in four genes overlapping. The functions of overlapping genes are widely. Conclusion We found that there are a lot of complex overlapping genes in HCMV Merlin strains, which are basis for further study of the transcription and translation mechanism of overlapping genes.
