Immunogenicity of sabin inactivated poliovirus vaccine induced by diphtheria-tetanus-acellular pertussis and Sabin inactivated poliovirus combined vaccine.
- Author:
Yan MA
1
;
Min QIN
;
Hui-Qiong HU
;
Guang JI
;
Ling FENG
;
Na GAO
;
Jie GU
;
Bing-Feng XIE
;
Ji-Hong HE
;
Ming-Bo SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; immunology; Diphtheria-Tetanus-acellular Pertussis Vaccines; immunology; Female; Male; Poliovirus Vaccine, Inactivated; immunology; Rats; Rats, Wistar; Vaccines, Combined; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(3):197-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to search the preparation process and optimazing dosage ratio of adsorbed diphtheria-tetanus-acellular pertussis and sabin inactivated poliovirus combined vaccine (DTaP-sIPV), the neutralizing antibody titers of IPV induced by different concentration of DTaP-sIPV were investigated on rats.
METHODSTwo batches of DTaP-sLPV were produced using different concentration of sIPV and the quality control was carried. Together with sabin-IPV and DTaP-wIPV ( boostrix-polio, GSK, Belgium) as control group, the DTaP-sIPV were administrated on three-dose schedule at 0, 1, 2 month on rats. Serum sample were collected 30 days after each dose and neutralizing antibody titers against three types poliovirus were determined using micro-neutralization test.
RESULTSTwo batches of prepared DTaP-sIPV and control sLPV were according to the requirement of Chinese Pharmacopoeia (Volume III, 2005 edition) and showed good stability. The seropositivity rates were 100% for sabin inactivated poliovirus antigen in all groups. The GMTs (Geometric mean titers) of neutralizing antibodies against three types poliovirus increased.
CONCLUSIONThe prepared DTaP-sIPV was safe, stable and effective and could induced high level neutralizing antibody against poliovirus on rats.
