Immunogenicity of Sabin inactivated poliovirus vaccine induced by diphtheria-tetanus-acellular pertussis and Sabin inactivated poliovirus combined vaccine
10.3760/cma.j.issn.1003-9279.2011.03.013
- VernacularTitle:DTaP-sIPV联合疫苗中SabinIPV 免疫原性研究
- Author:
Yan MA
1
;
Min QIN
;
Hui-Qiong HU
;
Guang JI
;
Ling FENG
;
Na GAO
;
Jie GU
;
Bing-Feng XIE
;
Ji-Hong HE
;
Ming-Bo SUN
Author Information
1. 中国医学科学院北京协和医学院
- Keywords:
Poliovirus vaccine,inactivated;
Antibodies,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2011;25(3):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to search the preparation process and optimazing dosage ratio of adsorbed diphtheria-tetanus-acellular pertussis and sabin inactivated poliovirus combined vaccine ( DTaPsIPV) , the neutralizing antibody titers of IPV induced by different concentration of DTaP-sIPV were investigated on rats. Methods Two batches of DTaP-sIPV were produced using different concentration of sIPV and the quality control was carried. Together with sabin-IPV and DTaP-wIPV ( boostriixTM-polio, GSK, Belgium) as control group,the DTaP-sIPV were administrated on three-dose schedule at 0,1,2 month on rats. Serum sample were collected 30 days after each dose and neutralizing antibody titers against three types poliovirus were determined using micro-neutralization test. Results Two batches of prepared DTaP-sIPV and control sIPV were according to the requirement of Chinese Pharmacopoeia ( Volume Ⅲ , 2005 edition) and showed good stability. The seropositivity rates were 100% for sabin inactivated poliovirus antigen in all groups. The GMTs( Geometric mean titers) of neutralizing antibodies against three types poliovirus increased. Conclusion The prepared DTaP-sIPV was safe, stable and effective and could induced high level neutralizing antibody against poliovirus on rats.
