Analysis of drug-resistant multi-loci mutation of P area of HBV genome in 32 patients with chronic hepatitis B.
- Author:
Fei YE
1
;
Xiao-Ou LI
;
Jian-Chun GUO
;
Jing WU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Drug Resistance, Viral; genetics; Female; Genome, Viral; genetics; Hepatitis B virus; genetics; Hepatitis B, Chronic; virology; Humans; Male; Mutation; genetics; Polymerase Chain Reaction
- From: Chinese Journal of Experimental and Clinical Virology 2011;25(3):208-210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to deeply understand the effects of nucleoside analogues to HBV DNA polymerase area, which initiate a state of drug resistance due to HBV genome mutation.
METHODSUsing PCR product direct sequencing method to test the gene sequence of P area of HBV genome of the patients who taking nucleoside analogues and showing signs of virologic breakthrough.
RESULTSMore resistance mutations can be find in HBV DNA polymerase area, which causing clinically cross drug resistance and multiple resistance.
CONCLUSIONWe should choose higher genetic barrier for the initial treatment. And the patients long-term using of nucleoside analogues, should be regularly monitored serum HBV DNA level, in order for early detection of virological breakthrough and timely intervention.
