Human cytomegalovirus glycoprotein B genotypes in congenitally infected neonates

VernacularTitle:先天性巨细胞病毒感染新生儿gB基因分型研究
Author: Min-Gang ZHANG ¹ ; Heng-Bing WANG ; Yan-Zhou WANG ; Qi PAN
Author Information

1. 山东大学附属省立医院
Keywords: Cytomegalovirus infections; Genotype; Polymerase chain reaction; Polymorphism,restriction fragment length
From: Chinese Journal of Experimental and Clinical Virology 2011;25(4):262-264
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate human cytomegalovirus (HCMV) glycoprotein B (gB)genotypes and clinical features in neonates with congenital infections. MethodsUrine samples were obtained from 67 neonates with HCMV infection confirmed by polymerase chain reaction (PCR). The gB gene fragment was amplified by nested PCR. HCMV gB genotyping was detected by restriction fragment length polymorphism. ResultsIn all these cases, the most prevalent genotype was gB1 (50. 7％ ),followed by gB3 (23.9％), gB2 ( 17.9％ ), and gB1/gB3 coinfection ( 7.5％ ) ; gB4 was not found. Moreover, gB1 was more prevalent in infants with liver damage (27/37,73.0％) than in other symptomatic infants without liver damage ( 13/30, 43.3％ ; P ＜ 0. 05 ). ConclusionThe gB1 genotype is the most prevalent in infants with congenital symptomatic HCMV disease, especially in those with liver damage, followed by genotypes gB3, gB2, and gB4.