Sequencing and analyses of the adenovirus polymerase gene in fecal samples of captively bred rhesus macaques

VernacularTitle:恒河猴肠道腺病毒聚合酶基因片段的序列分析
Author: Jian-Xi LU ¹ ; Hong CAO ; Shi-Yu QIAN ; Qiang WANG ; Gang LI
Author Information

1. 中山大学附属第三医院
Keywords: Macaco mulata; Adenovirus,simian; Genes
From: Chinese Journal of Experimental and Clinical Virology 2011;25(4):268-270
CountryChina
Language:Chinese
Abstract: Objective In an attempt to study the moleculr characterization and epidemiology of simian adenoviruses in nonhuman primate (NHP) populations. MethodsWe examined a colony of captively bred rhesus macaques (Macaca mulatta ) in China for the presence of adenoviral DNA in stool samples. This was done by using the PCR method that targeted the adenovirus polymerase gene, and the PCR positive fragments were cloned for sequencing and phylogenetic analyses. ResultsAmong the 57animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA. The results suggested that the viral DNA clones were primarily segregated into two large groups: SAdV-6 (2 nonredundant sequences) and SAdV-7 (9 non-redundant sequences). In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively. ConclusionOur data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.