Preparation of monoclonal antibodies against oh(8)dG and their biological characteristics.
- Author:
Jian WANG
1
;
Jian-Gen SHEN
;
Yong-Liang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; Blotting, Western; Deoxyguanosine; analogs & derivatives; analysis; immunology; Enzyme-Linked Immunosorbent Assay; Female; Gastric Mucosa; chemistry; Helicobacter Infections; diagnosis; Helicobacter pylori; Immunohistochemistry; Mice; Mice, Inbred BALB C
- From: Journal of Zhejiang University. Medical sciences 2003;32(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare monoclonal antibodies against oh(8)dG and to evaluate the relationship between Hp infection and oxidative DNA damage by detecting oh8dG in gastric mucosa.
METHODSBALB/C mice were immunized with BSA-oh(8)dG conjugate, monoclonal antibodies were prepared by hybridoma technique, the biological characteristics of antibodies were analysed by competitive ELISA, Western blot and immunohistochemistry.
RESULTSTwo strains of hybridoma cell were obtained. ELISA and Western blot indicated that the antibodies were fairly specific for oh(8)dG. In immunohistochemistry,the positive rate of oh(8)dG expression in Hp positive tissues and Hp negative tissues was 55% and 5%, respectively(P<0.01).
CONCLUSIONThe prepared antibodies can specially recognize oh(8)dG and immunohistochemistry with the monoclonal antibodies showed Hp infection can increase oh(8)dG level in gastric mucosa.
