Improvement of cardiomyocyte isolation for different purposes.
- Author:
Chun-Mei CAO
1
;
Xiong ZHANG
;
Ying-Ying CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Action Potentials; Animals; Calcium Channels; physiology; Cell Separation; methods; Female; Heart Ventricles; cytology; Male; Myocytes, Cardiac; physiology; Potassium Channels; physiology; Rats
- From: Journal of Zhejiang University. Medical sciences 2003;32(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo improve the methods of isolating single ventricular myocytes for purposes of different experiments.
METHODSThe cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion technique at constant flow rate. The patch clamp whole-cell recording, a spectrofluorometric method and a video tracking system were used to verify the basic electrophysiological properties, intracellular calcium transient and cell contraction of the single myocytes.
RESULTSThe resting membrance potential of the myocyte was (-74.06+/-4.54)mV. The whole-cell sodium, potassium and calcium currents, intracellular calcium transients and contractile properties were stable and typical.
CONCLUSIONThe myocytes obtained by adjusting perfusion parameters are well suited for electrophysiological recording, intracellular calcium and single cell contraction measurements.
