Effect of HIV-1 infection on apoptosis of CD4+ T lymphocytes mediated by Fas.
- Author:
Nan-ping WU
1
;
Dan LI
;
Armin BADER
;
Stefan HOXTERMANN
;
Norbert BROCKMEYER
Author Information
- Publication Type:Journal Article
- MeSH: Acquired Immunodeficiency Syndrome; immunology; Adult; Apoptosis; CD4-Positive T-Lymphocytes; pathology; Female; HIV-1; Humans; Leukocyte Common Antigens; blood; Male; Middle Aged; fas Receptor; blood; physiology
- From: Journal of Zhejiang University. Medical sciences 2003;32(2):90-93
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the pathogenic role of Fas/CD95 in HIV-1 infection subjects, and to investigate the effects of HIV on plasma levels of sFas and the expression of CD95 on different CD4(+) T lymphocyte subpopulations.
METHODSFour-color flow cytometry was used to determine the expression of CD95, CD45RO, CD45RA on CD4(+ )T lymphocyte in peripheral blood from HIV-1 infection subjects and serum Fas levels were quantified by enzyme-linked immunosorbent assay (ELISA).
RESULTCompared with healthy controls, serum Fas levels were significantly increased (P<0.05) in HIV group and positively correlated with the disease progress. The expression of CD95 on naive T-lymphocyte subsets was increased whereas that on memory T-lymphocyte subsets was decreased.
CONCLUSIONFas plays an important role in the deletion of CD4(+) T-lymphocyte during HIV-1 infection. Further understanding of the relationship between Fas/CD95 and CD45RO/CD45RA may help to predict the progression of the disease and the clinical outcome.
