Isolation, culturation and adipogenisis committed differentiation of adult human mesenchymal stem cell.
- Author:
Hang-hui CEN
1
;
Chun-mao HAN
;
Ping-ping LAI
;
Qing-Hua LU
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; physiology; Adult; Antigens, CD34; analysis; Bone Marrow Cells; physiology; Cell Differentiation; Cell Separation; methods; Cells, Cultured; HLA-DR Antigens; analysis; Humans; Hyaluronan Receptors; analysis; Lipopolysaccharide Receptors; analysis; Stem Cells; physiology
- From: Journal of Zhejiang University. Medical sciences 2003;32(2):137-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate MSCs from adult human bone marrow cells and to induce them into adipocytes.
METHODSMSCs were isolated from adult human bone marrow aspirated by Percoll and expanded in L-DMEM. The surface antigen of MSCs, CD14, CD34, CD45, CD44, VLA-1, HLA-DR and cell cycle were analysed on a FACScan flow cytometer. MSCs were cultured in adipogenisis inducing medium including insulin, 1-methyl-3-isobutylxanthine, indomethine and dexamethasone for 7 days and stained with Oil Red O.
RESULTSMSCs grew as adherent cells and expanded more than 10 passages. They were positive for CD44 and negative for CD14, CD34, CD45, HLA-DR. The expression of VLA-1 was weak. After 7 days of adipocyte inducing, about 85%of the cells displayed accumulation of lipid vacuoles, as detected by Red Oil O.
CONCLUSIONMSCs isolated and cultured from adult human bone marrow can be induced to adipogenisis committed differentiation.
