Role of interleukin-2 in the functional myocardial impairment induced by anoxia and reoxygenation.
- Author:
Chun-mei CAO
1
;
Hui YAO
;
Wan-hong XU
;
Zhi-guo YE
;
Jun-zhu CHEN
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Interleukin-2; physiology; Lipid Peroxidation; Male; Malondialdehyde; blood; Mitochondria, Heart; metabolism; Myocardial Ischemia; physiopathology; Myocardial Reperfusion Injury; physiopathology; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2003;32(3):175-180
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of interleukin-2 (IL-2) on myocardial impairment during ischemia/reperfusion or anoxia/reoxygenation.
METHODSChemical anoxia was introduced in the isolated rat ventricular myocytes by Krebs-Henseleit (K-H) solution containing 10(-3) mol/L sodium dithionite. The video-tracking system and spectrofluorometric method were employed to verify the cell contraction and calcium homeostasis of the single myocyte. Radioimmunoassay was used to analyze the IL-2 levels in myocardium.
RESULTSThe levels of IL-2 in myocardium subjected to ischemia/reperfusion were elevated [(14.34+/-5.99 compared with 22.25+/-3.68)ng/g, P<0.01]. During anoxia, cell contraction and the amplitude of electrically induced calcium transient were depressed and the parameters did not return to the pre-anoxia level during reoxygenation. IL-2 at 200 U/L administered during anoxia aggravated the effect of reoxygenation on cell contraction and calcium transient. After perfusion with IL-2, the malondialdehyde content of myocardial mitochondria was elevated.
CONCLUSIONCoexistence of IL-2 during anoxia aggravates the effect of reoxygenation on the cell contraction and calcium homeostasis in the isolated rat ventricular myocytes, in which the mitochondrial lipid peroxidation induced by IL-2 is involved.
