Treatment of interferon-alpha in reducing the endothelium-dependent relaxation of rat thoracic aorta.
- Author:
Hui YAO
1
;
Chun-mei CAO
;
Hong-feng JIN
;
Qi-xian SHAN
;
Lin-lin WANG
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcholine; pharmacology; Animals; Aorta, Thoracic; drug effects; physiology; Endothelium, Vascular; physiology; Guanylate Cyclase; physiology; Interferon-alpha; pharmacology; Male; Nitric Oxide; physiology; Nitric Oxide Synthase; physiology; Nitric Oxide Synthase Type II; Phenylephrine; pharmacology; Rats; Rats, Sprague-Dawley; Vasodilation; drug effects
- From: Journal of Zhejiang University. Medical sciences 2003;32(3):202-206
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the vascular effect of acute and chronic treatment of interferon-alpha (IFN-alpha) in rat aortic rings.
METHODSIsolated thoracic aortic rings were mounted on the organ bath and the tension of the vessel was recorded.
RESULTSIFN-alpha(10, 100, 1,000 and 10,000 U/ml) caused concentration -dependent relaxation of endothelium-intact aorta rings preconstricted with phenylephrine (PE,10(-6)mol/L), to(90.1+/-0.91)%, (65.1+/-5.21)%, (39.5+/-8.22)% and (35.3+/-8.27)% of pre-drug control, respectively. Removal of the endothelium inhibited the relaxation by IFN-alpha. The vasorelaxant effect of IFN-alpha (100 U/ml ) was attenuated by pretreatment with L-NAME (10(-4)mol/L), methylene blue (10(-5)mol/L) or AMG (10(-4)mol/L), to (97.2+/-5.34)%, (95.1+/-6.25)% and (93.7+/-8.82)% of the control, respectively. Pretreatment with IFN-alpha (1,000,000 U/d, i.p.) for five days markedly inhibited the endothelium-dependent relaxation of the aortic rings to acetylcholine. But the endothelium-dependent relaxation to acetylcholine was not changed by pretreatment of IFN-alpha (10,000 U/ml) with the isolated aorta rings for 2 h.
CONCLUSIONThe vasorelaxation induced by IFN-alpha in rat aorta rings is endothelium-dependent and is possibly mediated by inducible nitric oxide synthase. Chronic treatment of IFN-alpha may impair the endothelium or NO-sGC pathway.
