Increase of LTB4 level and expression of LTA4-hydrolase mRNA in lung tissue and cerebral cortex in asthmatic rats.
- Author:
Yang-mei DENG
1
;
Qiang-min XIE
;
Ji-qiang CHEN
;
Jun-fang DENG
;
Ru-lian BIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Asthma; metabolism; Cerebral Cortex; chemistry; metabolism; Epoxide Hydrolases; genetics; Female; Leukotriene B4; analysis; Lung; chemistry; metabolism; Male; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2003;32(4):296-322
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate antigen-induced changes of leukotriene B(4)(LTB(4))content and LTA(4)-hydrolase mRNA expression in lung tissue and cerebral cortex in sensitized rats.
METHODSThe contents of LTB(4) in lung tissue and cerebral cortex homogenates and LTA(4)-hydrolase mRNA expression after antigen challenge by aerosol were respectively detected by reverse-phase high performance liquid chromatography(RP-HPLC) and semi-quantitative RT-PCR.
RESULTThe LTB(4) levels in lung tissue and cerebral cortex homogenates in asthmatic rats were significantly higher than those in control (P%0.05), and LTA4-hydrolase mRNA expression was also increased in asthmatic group. Dexamethason(DXM, 0.5 mg/kg, i.p.) decreased the LTB(4) content and inhibited the LTA(4)-hydrolase mRNA expression significantly in asthmatic rats(P%0.05).
CONCLUSIONLTB(4) content and LTA(4)-hydrolase mRNA expression in cerebral cortex and lung tissue are increased in asthmatic rats, and there may exist neuroimmunological cross-talking between central nervous system and lung tissue in asthma.
