Proteomic analysis of cellular responses to low concentration of N-methyl-N'-nitro-N-nitrosoguanidine in human amnion FL cells.
- Author:
Jing-hua JIN
1
;
Zhi-hua GAO
;
Jun YANG
;
Ying-nian YU
Author Information
- Publication Type:Journal Article
- MeSH: Amnion; chemistry; cytology; drug effects; Cells, Cultured; Electrophoresis, Gel, Two-Dimensional; Humans; Methylnitronitrosoguanidine; toxicity; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Journal of Zhejiang University. Medical sciences 2003;32(5):375-379
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protein profile after treatment of low concentration of N- methyl-N'-nitro-N-nitrosoguanidine (MNNG) in human FL cells.
METHODSAfter MNNG treatment, whole cellular proteins were separated using two-dimensional gel electrophoresis and visualized by silver staining; the digitized images were analyzed with 2D analysis software. The differentially expressed protein spots were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).
RESULTMore than 60 proteins showed significant changes in MNNG-treated cells compared to control cells (DMSO treatment). There were 18 protein spots detected only after MNNG treatment, while 13 protein spots were detected only in the control cells. Moreover, the levels of another 31 proteins were either increased or decreased in MNNG-treated FL cells. And some of the proteins were identified by MALDI-TOF-MS.
CONCLUSIONThere are significant alterations of protein profile after MNNG attack.
