Altered of zinc finger proteins expression in FL cells following benzo a pyrene treatment.
- Author:
Zhi-hua GAO
1
;
Jing-hua YANG
;
Jun YANG
;
Ying-nian YU
Author Information
- Publication Type:Journal Article
- MeSH: Amnion; chemistry; cytology; drug effects; Benzo(a)pyrene; toxicity; Cells, Cultured; DNA Repair; Electrophoresis, Gel, Two-Dimensional; Humans; Proteomics; Zinc Fingers
- From: Journal of Zhejiang University. Medical sciences 2003;32(5):380-384
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand benzo[a]pyrene (B[a]P) mediated cellular responses, and to provide clues to explore molecular mechanism of mutagenesis and carcinogenesis induced by B[a]P.
METHODSTwo-dimensional electrophoresis (2-DE) was used to investigate the protein expression levels of FL cells after B[a]P exposure, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify the differentially expressed proteins.
RESULTStatistical analysis showed that the volumes of 47 protein spots were altered after B[a]P treatment (P<0.05) and 23 proteins were successfully identified, including zinc finger proteins, SWI/SNF related protein, Bromo domain containing domain and other proteins.
CONCLUSIONThese affected proteins may be involved in the cellular responses to B[a]P exposure, and may mediate the B[a]P induced mutagenesis and carcinogenesis.
