Activation of transcription factors induced by low concentration of N-methyl-N'-nitro-N-nitrosoguanidine.
- Author:
Gu-liang WANG
1
;
Zheng WANG
;
Jun YANG
;
Ying-nian YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cercopithecus aethiops; Cyclic AMP Response Element-Binding Protein; drug effects; Methylnitronitrosoguanidine; toxicity; Mutation; NF-kappa B; drug effects; Proto-Oncogene Proteins c-myc; drug effects; Transcription Factor AP-1; drug effects; Transcription Factors; drug effects; Vero Cells
- From: Journal of Zhejiang University. Medical sciences 2003;32(5):390-392
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of MNNG on some of the transcription factors such as NF- kappaB, CREB, AP-1 and c-Myc.
METHODSThe activities of these transcription factors were measured by transient transfection assay of SEAP vectors.
RESULTThe expressions of AP-1, CREB and NF- kappaB driven reporter genes were elevated for about 1.3, 1.4 and 1.3 times in MNNG-treated cells, respectively, as compared to untreated controls. However, the exposure of MNNG had no effect on the activity of c-Myc.
CONCLUSIONThe activation of certain transcription factors might be involved in the process of untargeted mutation induced by low concentration of MNNG treatment.
