Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.
- Author:
Ping-yong YI
1
;
Hai YU
;
Wen-xue MA
;
Wen-jun WU
;
Huai JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Base Sequence; Enterotoxins; genetics; GPI-Linked Proteins; Isoenzymes; genetics; Molecular Sequence Data; Polymerase Chain Reaction; RNA Splicing; Recombinant Fusion Proteins; genetics
- From: Journal of Zhejiang University. Medical sciences 2003;32(5):412-414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.
METHODSThe SEA gene and a DNA fragment encoding the signal for GPI-anchor attachment of hPLAP -1 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric GPI- anchored SEA molecule with gene splicing by overlap extension. The resulting chimera was cloned in pGEM-T vector and verified by sequencing analysis.
RESULTA chimeric SEA-hPLAP-1 cDNA was successfully constructed with gene splicing by overlap extension.
CONCLUSIONGene splicing by overlap extension is a successful specific PCR technique for gene recombination.
