Cloning,expression and functional identification of secoisolariciresinol dehydrogenase gene from Dysosma versipellis callus.

Author: Yun SHEN ¹ ; Ri-Dao CHEN ¹ ; Ke-Bo XIE ¹ ; Jian-Hua ZOU ¹ ; Jun-Gui DAI ¹
Author Information

1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
Publication Type:Journal Article
Keywords: Dysosma versipellis; SDH; biosynthesis; podophyllotoxin
From: China Journal of Chinese Materia Medica 2016;41(24):4568-4571
CountryChina
Language:Chinese
Abstract: Secoisolariciresinol dehydrogenase (SDH) is a key enzyme involved in the biosynthetic pathway of podophyllotoxin.In this study, two SDH candidate genes,SO282 and SO1223, were cloned from callus of Dysosma versipellis by homology-based PCR and rapid amplification of cDNA end (RACE).The SDH candidate genes were expressed in Escherichia coli and the subsequent enzyme assay in vitro showed that recombinant SO282 had the SDH activity. These results pave the way to the follow-up investigation of the biosynthetic of podophyllotoxin.