Germplasm identification of Lonicerae Japonicae Flos by DNA melt curve analysis.

Author: Ti-Ying CHEN ¹ ; Chao JIANG ¹ ; Yuan YUAN ¹ ; Kang CHEN ¹ ; Jun-Hui ZHOU ¹ ; Yu-Yang ZHAO ¹ ; Lu-Qi HUANG ¹
Author Information

1. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: Lonicerae Japonicae Flos; germplasm identification; high resolution melting
From: China Journal of Chinese Materia Medica 2016;41(24):4572-4578
CountryChina
Language:Chinese
Abstract: To establish a new high resolution melting analytical method for identification of Lonicera japonica germplasm, the screening of 7 pairs of SSR (simple sequence repeats) primers, determining the suitable diagnostic primers by the differences of peak pattern and Tm was conducted. Then into the DNA template concentration, annealing temperature and the suitable range of cycle number were investigated. Combined with SIMCA-P software for data processing analysis, the results show that three main germplasm honeysuckle could be divided by four sets of primers. It provides methodology for improving L. japonica germplasm identification.