Optimization of alcohol precipitation technology of Biqiu granules based on index components and pharmacodynamics.
- Author:
Yan-Jun ZHANG
1
;
Li-Li LIU
1
;
Yun WU
1
;
Jun-Hua HU
1
;
Xiu-Juan CHANG
1
;
Wei XIAO
1
;
En-Xiang CHAO
2
Author Information
- Publication Type:Journal Article
- Keywords: Biuqiu granules; Box-Behnken design; Plackett-Burman design; alcohol precipitation process; pharmacodynamics
- From: China Journal of Chinese Materia Medica 2016;41(24):4598-4604
- CountryChina
- Language:Chinese
- Abstract: To evaluate the rationality of alcohol precipitation technology of Biqiu granule by investigating its effect on serum histamine, IgE, IL-4, IFN and TNF-α. The contents of cafferic acid and rosmarinic acid were used as the evaluation indexes, and some factors affecting index were firstly evaluated by Plackett-Burman design; then alcohol precipitation technology was further optimized by Box-Behnken design to determine the optimal alcohol precipitation conditions. The best alcohol precipitation conditions were as follows: the relative density of herb liquor was 1.15 (65 ℃); the concentration of alcohol was 70%, and standing time was 12 hours. Optimal alcohol precipitation technology of Biqiu granules determined by pharmacodynamic screening, Plackett-Burman and Box-Behnken design tests, was stable and feasible with good predictability, providing reliable basis for the industrialization production of Biqiu granules.
