Effects of Xuesaitong injection on inflammation induced by OGD/reoxygenation in BV2 microglia.
10.19540/j.cnki.cjcmm.20161222.029
- Author:
Qian HONG
1
;
Shi WANG
1
;
Chang-Xiu CHEN
1
;
Yu ZHANG
1
;
Yang WANG
1
;
Yuan PAN
1
;
Zeng-Chun MA
2
Author Information
1. No.97 Hospital of PLA, Xuzhou 221000, China.
2. Institute of Radiation Medicine Science, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- Keywords:
MAPK;
NF-κB p65;
OGD/R;
Xuesaitong(XST) injection;
pro-inflammatory mediator
- From:
China Journal of Chinese Materia Medica
2017;42(1):140-145
- CountryChina
- Language:Chinese
-
Abstract:
The present study aims to investigate the effects of Xuesaitong (XST)injection on inflammation induced by OGD/reoxygenation in BV2 microglia cell and explore the underlying mechanisms.The effects of XST injection were evaluated in terms of cell viability, secretion of TNF-α, IL-1β, IL-6 and IL-10 into culture media, protein expression of p-ERK1/2, p-JNK and p-p38, and nuclear translocation of NF-κB p65. The results showed that XST injection significantly increased cell viability, suppressed release of TNF-α, IL-1β, IL-6 and IL-10 and down-regulated p-JNK1/2 and p-p38 MAPK expression in BV2 microglia cells induced by OGD/R injury, whereas it had no effect on p-ERK1/2 expression. Furthermore, XST injection suppressed nuclear translocation of NF-κB p65 in BV2 microglia after OGD/R injury. These data indicate that the neuroprotective effects of XST injection on OGD/R injury are associated with its inhibition of pro-inflammatory mediator production, down-regulation of JNK1/2 and p38 MAPK activation, and suppression of NF-κB p65 nuclear translocation in BV2 microglia cells.
