Analysis and evaluation of eight active ingredients in Lilium lancifolium from different regions.
10.19540/j.cnki.cjcmm.20161222.035
- Author:
Huang-Qin ZHANG
1
;
Hui YAN
1
;
Da-Wei QIAN
1
;
Zhen-Hua ZHU
1
;
Sheng GUO
1
;
Lan-Ping GUO
2
;
Zhi-Shu TANG
3
;
Jin-Ao DUAN
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Shanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shanxi University of Chinese Medicine, Xianyang 712083, China.
- Publication Type:Journal Article
- Keywords:
Lilium lancifolium;
UPLC-TQ-MS;
correlation analysis;
determination;
principal components analysis
- From:
China Journal of Chinese Materia Medica
2017;42(2):311-318
- CountryChina
- Language:Chinese
-
Abstract:
This study established a rapid UPLC-TQ-MS/MS method for determination of eight active ingredients in Lilium lancifolium. The contents range of regaloside E, F, C and B are as follows: 0.604 0×10⁻¹-18.62×10⁻¹, 0.680 0×10⁻²-44.75×10⁻², 0.700 0×10⁻³-29.65×10⁻¹, 0.170 0×10⁻¹-4.724 mg•g⁻¹; the contents of chlorogenic acid, caffeic acid, protocatechualdehyde and ferulic acid, within the range of 6.827×10⁻³-16.07×10⁻³, 0.011 1×10⁻³-79.71×10⁻³, 0.593 7×10⁻³-2.962×10⁻³, 2.606×10⁻²-45.89×10⁻² mg•g⁻¹, respectively. According to PCA (principal components analysis) plotting, 35 batches can be divided into two categories, namely Anhui Huoshan and Hunan Longshan. The main different elements between these two categories are caffeic acid and ferulic acid according to the VIP (variable importance in the projection) points figure. Based on comprehensive principal component values, there are eight batches of L. lancifolium from Huoshan among the comprehensive ranking of ten. The UPLC-TQ-MS method for simultaneous analysis of eight active ingredients is accurate, efficient and convenient. This result can provide scientific basis for quality control of L. lancifolium.
