Anti-platelet adhesive effect and mechanisms of Xueshuantong capsules under flow conditions.
10.19540/j.cnki.cjcmm.20161222.014
- Author:
Shu-Xian HAN
1
;
Ying CHEN
1
;
Qian ZHANG
1
;
Bing HAN
2
;
Yi-Meng GE
2
;
Yan-Hua XIANG
2
;
Fu-Long LIAO
1
;
Yun YOU
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China.
2. Harbin Zhenbao Pharmaceutical Co., Ltd., Harbin 150060, China.
- Publication Type:Journal Article
- Keywords:
Shear stress;
Xueshuantong capsule;
platelets;
vascular endothelial cells
- From:
China Journal of Chinese Materia Medica
2017;42(2):341-346
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the anti-platelet adhesive effect and possible mechanisms of Xueshuantong capsule (XST) under flow conditions. Human umbilical vein endothelial cells (HUVECs) and human platelets were employed as experimental materials, and TNF-α (20 μg•L⁻¹) was used to establish vascular endothelial cell injury models. In vivo flow conditions were simulated under controlled shear stress of 0.1 Pa and 0.9 Pa by Bioflux1000 assays accordingly. Anti-platelet adhesive effects of XST at 0.3 g•L⁻¹ were dynamically monitored by microscopic time-lapse photography. Western blotting was employed to detect the VCAM-1 expression on endothelial cells, and the release of 6-keto-PGF1α and TXB2 was tested by radioimmunoassay. The results showed that XST could inhibit the platelets adhesion under both physiological and pathological flow conditions, and the inhibition rate was 15.0% and 34.1% respectively. Under pathological low shear stress or static conditions, XST could significantly inhibit endothelial cells VCAM-1 expression and TXB2 release (P<0.05). These results suggested that XST inhibited platelets adhering to injured endothelium via decreasing VCAM-1 expression and TXA2 secretion from endothelium. From the interactions among blood flow, vascular endothelium and platelets, the anti-thrombosis effects of XST were possibly related to endothelial cells protection and therefore inhibiting platelets adhesion. Under different flow conditions, the antiplatelet adhesion effect of XST was different, and the pathological low shear stress was more conducive to the efficacy of XST.
