Effect of processed Polygonum multiflorum on mRNA expression level of five subtypes of CYP450 enzymes in rat liver.
10.19540/j.cnki.cjcmm.20161222.079
- Author:
Chun-Lian HUANG
1
;
Xue-Mei FAN
2
;
Qian LI
3
;
Yi-Ming WANG
2
;
Shu-Mei WANG
1
;
Meng-Juan GONG
1
;
Guo-An LUO
1
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
2. Department of Chemistry, Tsinghua University, Beijing 100084, China.
3. Guangzhou Consun Pharmaceutical Co., Ltd., Guangzhou 510610, China.
- Publication Type:Journal Article
- Keywords:
CYP450;
liver injury;
mRNA expression;
processed Polygonum multiflorum;
serum biochemical indicators
- From:
China Journal of Chinese Materia Medica
2017;42(2):352-356
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect of processed Polygonum multiflorum on mRNA expression levels of five subtypes of CYP450 enzymes in rat liver. SD rats were randomly divided into the normal control group, processed P. multiflorum high dose and low dose groups (5.40 g•kg⁻¹ and 1.08 g•kg⁻¹). The rats in administration groups were continuously given with processed P. mutiflorum for 7 days by ig administration, and the rats in normal control group were given with the same volume of distilled water. After successive administration of 7 days, the serum biochemical indications were detected, and Real-time quantitative PCR technology was used to detect the mRNA expression levels of five subtypes of CYP450 enzymes in rat liver. Experimental results showed that AST was decreased significantly in both low and high dose groups. ALT was significantly decreased in low dose group and significantly increased in high dose group. The mRNA expression levels of five subtypes of CYP450 enzymes in rat liver were decreased in high dose and low dose groups in a dose-dependent manner. Especially the high dose processed P. multiflorum could significantly inhibit CYP1A2 and CYP2E1 mRNA expression levels in rats. The study showed that high dose P. multiflorum water extract had hepatotoxicity, and the degree of liver damage was increased with the increase of dose. It shall be noted that 5.40 g•kg⁻¹ water extract of P. multiflorum could significantly inhibit CYP1A2 and CYP2E1 mRNA expression levels in the liver of rats.
