Anti-inflammation effect of Jinlingzi San in rat metabonomics based on 1H-NMR and LC-MS technology.
10.19540/j.cnki.cjcmm.20161222.028
- Author:
Shu-Jie SHEN
1
;
Su-Fang SHUI
2
;
Bing-Kun XIAO
1
;
Jian-Yun YANG
1
;
Rong-Qing HUANG
1
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
2. Anhui Medical University, Hefei 230032, China.
- Publication Type:Journal Article
- Keywords:
Jinlingzi San;
LC-MS;
anti-inflammation;
metabonomics
- From:
China Journal of Chinese Materia Medica
2017;42(2):363-369
- CountryChina
- Language:Chinese
-
Abstract:
To further explore the regulatory effect of Jinlingzi San on in vivo inflammatory mechanism during inflammatory treatment, this study adopted 1H-NMR and LC-MS technology to analyze differences in in vivo metabolites of carrageen-induce rat foot swelling model. Besides, biomarkers related to inflammation models of Jinlingzi San in SD rats were discovered to speculate the regulatory mechanism of Jinlingzi San in resisting carrageen-induce inflammation. Through the analysis of detection spectrum, we found 18 biomarkers of metabolites(citrate, pyruvate, malic acid, succinate, glutamate, lysine, tartrate, 2-oxobutyric acid, glycine, guanosine, 9-cis-retinoic acid, triphosphate, inosine 5'-diphosphate, inosine diphosphate, tripolyphosphate, inorganic triphosphate, glycerophosphocholine, 21-deoxycortisol). Relevant pathway analysis results were TCA cycle, pyruvate metabolism, glycine, serine and threonine metabolism, and dicarboxylic acid metabolism. From the metabolic network, we can see that the anti-inflammatory effect of Jinlingzi San can regulate citric acid, succinic acid and glycine content to resist oxygen free radical and reduce body damage by ROS, so as to down-regulate inflammatory factors generated from body tissues and resist inflammation.
