Application of immunoassay in fast quality evaluation of traditional Chinese medicine.
10.19540/j.cnki.cjcmm.20170103.018
- Author:
Bo ZHANG
1
;
Tie-Gui NAN
2
;
Qing SUN
1
;
Hong-Mei LIU
1
;
Feng-Wei DING
3
;
Ying WANG
1
Author Information
1. College of Pharmacy, Linyi University, Linyi 276000, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Shandong C. P. Freda Pharm Co. Ltd., Ji'nan 250104, China.
- Publication Type:Journal Article
- Keywords:
ELISA;
colloidal gold immune test strip;
fast detection;
quality evaluation
- From:
China Journal of Chinese Materia Medica
2017;42(3):420-427
- CountryChina
- Language:Chinese
-
Abstract:
The quality evaluation of traditional Chinese medicine can be divided into two aspects, effectiveness and safety. The existing methods for evaluating the quality of traditional Chinese medicine(TCM) are mostly based on the testing instruments, which can not meet the practical needs of simple, rapid and on-site in production and life. Immunoassay, characterized by simple, rapid, sensitive, specific, low-cost and high-throughput, is widely used in the fields of clinical diagnosis, environmental pollution monitoring, food safety testing, and other fields. In recent years, immunoassay technology has been gradually applied in the field of quality control of TCM, involving quantitative detection of effective components of TCM, detection of harmful substances in TCM, and detection of exogenous pollutants in TCM. This paper summarizes the principle of the wide application of ELISA and colloidal gold immunostrip technology and its application in quality evaluation of TCM, this technique has a good application prospect in the field of rapid detection of the quality of TCM. In this paper, the principles of the widely used ELISA and colloidal gold immune test strip technology as well as their application in quality evaluation of TCM were reviewed, and the results showed that this technique had a good application prospect in the field of rapid detection of the quality of TCM.
