Comparison of Positron Emission Tomography Using 2-18F-fluoro-2-deoxy-D-glucose and 3-deoxy-3-18F-fluorothymidine in Lung Cancer Imaging.
- Author:
Fu-Li WANG
1
;
Ye-Ying TAN
2
;
Xiang-Min GU
1
;
Tian-Ran LI
3
;
Guang-Ming LU
4
;
Gang LIU
5
;
Tian-Long HUO
6
Author Information
- Publication Type:Journal Article
- MeSH: A549 Cells; Animals; Fluorodeoxyglucose F18; analysis; Humans; Lung Neoplasms; diagnostic imaging; Mice; Mice, Inbred BALB C; Mice, Nude; Positron Emission Tomography Computed Tomography; methods; Solitary Pulmonary Nodule; diagnostic imaging; Tomography, X-Ray Computed
- From: Chinese Medical Journal 2016;129(24):2926-2935
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDThe detection of solitary pulmonary nodules (SPNs) that may potentially develop into a malignant lesion is essential for early clinical interventions. However, grading classification based on computed tomography (CT) imaging results remains a significant challenge. The 2-[18F]-fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET)/CT imaging produces both false-positive and false-negative findings for the diagnosis of SPNs. In this study, we compared 18F-FDG and 3-deoxy-3-[18F]-fluorothymidine (18F-FLT) in lung cancer PET/CT imaging.
METHODSThe binding ratios of the two tracers to A549 lung cancer cells were calculated. The mouse lung cancer model was established (n = 12), and micro-PET/CT analysis using the two tracers was performed. Images using the two tracers were collected from 55 lung cancer patients with SPNs. The correlation among the cell-tracer binding ratios, standardized uptake values (SUVs), and Ki-67 proliferation marker expression were investigated.
RESULTSThe cell-tracer binding ratio for the A549 cells using the 18F-FDG was greater than the ratio using 18F-FLT (P < 0.05). The Ki-67 expression showed a significant positive correlation with the 18F-FLT binding ratio (r = 0.824, P< 0.01). The tumor-to-nontumor uptake ratio of 18F-FDG imaging in xenografts was higher than that of 18F-FLT imaging. The diagnostic sensitivity, specificity, and the accuracy of 18F-FDG for lung cancer were 89%, 67%, and 73%, respectively. Moreover, the diagnostic sensitivity, specificity, and the accuracy of 18F-FLT for lung cancer were 71%, 79%, and 76%, respectively. There was an obvious positive correlation between the lung cancer Ki-67 expression and the mean maximum SUV of 18F-FDG and 18F-FLT (r = 0.658, P< 0.05 and r = 0.724, P< 0.01, respectively).
CONCLUSIONSThe 18F-FDG uptake ratio is higher than that of 18F-FLT in A549 cells at the cellular level. 18F-FLT imaging might be superior for the quantitative diagnosis of lung tumor tissue and could distinguish lung cancer nodules from other SPNs.
